1. Protocols

Cell Culture Protocols  

Freezing Cells  

Passing Cells  

Simple purification of adenovirus  

Thawing Cells  

Transfection  

2. Materials

Cell Culture Dish  

Cell Culture Medium  

Coat Glass Bottom Dish with Fibronectin  

HEPES buffer  

1. Competent Cell Preparation

Competent Cell Preparation  

Electro-competent  

2. Transformation

Transformation Procedure  

3. Amplification

a. Miniprep

- DNA Amplification Mini Prep  

b. Maxiprep

- Detailed_Maxi_Prep_Procedure  

- DNA Amplification Maxi Prep  

Measure DNA Concentration  

Shipping and Receiving Plasmids on Filter Paper  

4. Primer Design

Primer design concerns  

5. PCR and Cloning Strategies

Colony_PCR  

Deletion and Addition Inserts  

Multiple mutations  

PCR Programs  

PCR_reaction_protocol  

Standard PCR  

6. Digestion

DNA Digestion  

7. Gel Purification

8. Ligation

Ligation of reporter to Adenovirus Ad5  

Ligation Samples  

9. Miscellaneous

general Molecular cloning Protocols  

1. Transfection

Lipofectimine 2000  

Transfection Proteins Ras and Src Treatment  

2. Immunoprecipitation and Immunoblotting

Immunoprecipitation and Immunoblotting  

3. Immunostaining

biotin_avidin_blocking  

Immunostaining General Protocol  

Immunostaining of Biotin-Streptavidin System  

Staining_biotin  

4. Constructs

c-Src Mutations  

Hamster c-ha-ras cds  

Membrane_binding_domain_review  

PH_domain_GFPs  

PIPs_Demirovic  

5. Miscellaneous

Antibodies  

Clustering of Epherin  

Coat Beads with Fibronectin  

CollagenConjugateRhodamin  

Inhibitors  

Integrin Antibodies  

Lysis Buffer  

Pervanadate  

Protocols for coupling carboxyl beads to EGF or Fibronectin  

Purify Protein Invitro Kinase Assay  

RGD Peptide Inhibitions  

Stimulation of Integrins  

Coupling_protein_carboxyl_beads  

Proteasephosphatase  

FPLC protocol  

His tag protein purification  

1. Microscope start up and shut down procedures

Confocal Microscopy Usage  

Rules and Tips for the Microscope Operation  

Starting Protocol for Nikon Microscope  

Startup_Protocol_for_Zeiss_scope  

2. Microscope Optical Setting and Diagrams

DIC microscope setting  

Nikon Microscope Parameters  

Zeiss Microscope Parameters  

3. Live Cell Imaging

Agarose Gel Protocol  

Cell_Imaging_Preparation_General_Procedure  

EDTA Solution  

EGF Experiment  

FRAP experiment protocol  

FRET related techniques  

Protocol of preparing adhesion assay imaging  

4. Miscellaneous

Alignment of Microscopy  

Conversions  

Kohler Alignment for Nikon  

microscope_cartoon(pptx)  

microscope_cartoon(pdf)  

5. Nikon

20130926 photoactivation Microscope settings-1  

illumination_control_1213_2013  

NikonMicroscopeParameters  

NikonMicroscopeParameters_old  

20130926 photoactivation Microscope settings-1  

illumination_control_1213_2013  

Nikon measurement and adjustment 2013Oct9  

nikon_neutrodensity_shutter(figure)  

nikon_neutrodensity_shutter(pptx)  

NK92_cell_culture  

NK92_cell_transfection  

CoatingQD-COOHonglassslides  

PDMS Training  

Qian_TC_micropattern_related_2010  

Silicon Master_Micro-Chem  

lab_training_modules  

Live Cell Imagine module  

recover_plasmid_from_filter_paper  

20130926 OM900 Manual