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  Molecular Engineering for Cellular Imaging and Reprogramming


  1. Peng Q., Huang Z. Sun K., Liu Y., Yoon C.W., Harrison R.E.S., Schmitt D.L., Zhu L., Wu Y., Tasan I., Zhao H., Zhang J., Zhong S., Chien S., and Wang Y. (2022) Engineering Inducible Biomolecular Assemblies for Genome Imaging and Manipulation in Living Cells, Nature Communications, Accepted
  2. Liu L., Qu Y., Cheng L., Yoon C.W., He P., Monther A., Guo T., Chittle S., and Wang Y. (2022) Engineering CAR-T Cells for Solid Tumor Therapy, Clinical and Translational Medicine, Accepted
  3. Yoon C.W., Pan Y., and Wang Y. (2022) The Application of Mechanobiotechnology for Immuno-engineering and Cancer Immunotherapy, Frontiers in Cell and Developmental Biology, doi: 10.3389/fcell.2022.1064484.
  4. Seong J., and Wang Y. (2022) Editorial: Visualization of molecular dynamics in live cells by fluorescent protein-based biosensors, Frontiers in Cell and Developmental Biology, PMID: 36313544
  5. Duan Y., Chen J., Meng X., Liu L., Shang K., Wu X., Wang Y., Huang Z, Liu H., Huang Y., Zhou C., Gao X., Wang Y., Sun J. (2022) Balancing activation and costimulation of CAR tunes signaling dynamics and enhances therapeutic potency, Molecular Therapy, PMID: 36045585, Accepted
  6. Lee HN, Hyeon SJ, Kim H, Sim KM, Kim Y, Ju J, Lee J, Wang Y, Ryu H, Seong J. (2022) Decreased FAK activity and focal adhesion dynamics impair proper neurite formation of medium spiny neurons in Huntington's disease, Acta Neuropathol. 2022 Jul 20. doi: 10.1007/s00401-022-02462-z
  7. Pan Y., Shi L.Z., Preece D., Gomez-Godinez V., Woo S.-H., Lu S., Wu Y., Chien S., Berns M.W., Liu L. & Wang Y. (2022) Mechanosensor Piezo1 Mediates Bimodal Patterns of Intracellular Calcium and FAK Signaling, EMBO Journal, Accepted
  8. Song Y., Soto J., Chen B., Hoffman T., Zhao W., Zhu N., Peng Q., Liu L., Ly C., Wong P.K., Wang Y., Rowat A., Kurdistani S., and Li S. (2022) Transient nuclear deformation primes epigenetic state and promotes cell reprogramming, Nature Materials, Accepted
  9. Kim H.S., Suh J.S., Jang Y.K., Ahn S.H., Choi G.H., Yang J.Y., Lim G.H., Jung Y., Jiang J., Sun J., Suk M., Wang Y., Kim T.J. (2022) Förster Resonance Energy Transfer-Based Single-Cell Imaging Reveals Piezo1-Induced Ca2+ Flux Mediates Membrane Ruffling and Cell Survival, Frontiers in Cell and Developmental Biology, 10:865056
  10. Wu Y., Huang Z., Harrison R., Liu L., and Wang Y. (2022) Engineering CAR T cells for enhanced efficacy and safety, APL Bioengineering (Featured Article), Accepted
  11. Wu H., Amirfakhri S., Hollandsworth H., Filemoni F., Liu Y., Lin H.H., Wu Y., Li Y.S., Chien S., Bouvet M., and Wang Y. (2022) Monocytes Engineered with iSNAP Inhibit Human B-Lymphoma Progression, Bioengineering & Translational Medicine, Accepted


  1. Gong Y., Wei C., Cheng L., Ma F., Lu S., Peng Q., Liu L. and Wang Y. (2021) Tracking the Dynamic Histone Methylation of H3K27 in Live Cancer Cells, ACS Sensors, 6(12):4369-4378
  2. Murphy K.J., Reed D.A., Vennin C., Conway J.R.W, Nobis M., Yin J., Chambers C.R., Pereira B.A., Lee V., Filipe E.C., Lucas M.C., Warren S.C., Skhinas J.N., Magenau A., Metcalf X.L., Stoehr J., Major G., Parkin A., Bidanel R., Zaratzian A., Silva A.D., Abdulkhalek L., Australian Pancreatic Genome Initiative (APGI), Gill A.J., Johns A.L., Biankin A.V., Samra J., Chou A., Burgess A., Caldon C.E., Horvath L., Daly R.J., Gadegaard N., Wang Y., Sansom O.J., Morton J.P., Cox T.R., Pajic M., Herrmann D. and Timpson P. (2021) Merlin status guides epithelial versus stromal targeting of FAK in pancreatic cancer: improving outcome in personalized medicine, Science Advances, 7(40):eabh0363
  3. Liu L., Limsakul P. Meng X., Huang Y., Harrison R.E.S., Huang T.-S., Shi Y., Yu Y., Charupanit K., Zhong S., Lu S., Zhang J., Chien S., Sun J. and Wang Y. (2021) Integration of FRET and Sequencing to Engineer Kinase Biosensors from Mammalian Cell Libraries for Drug Discovery, Nature Communications, 12(1):5031
  4. Wu Y., Liu Y., Huang Z., Wang X., Jin Z., Li J., Limsakul P., Zhu L., Allen M., Pan Y., Bussell R., Jacobson A., Liu T., Chien S., and Wang Y. (2021) Acoustogenetic Control of CAR T Cells via Focused Ultrasound, Nature Biomedical Engineering (Cover Article), 5(11):1336-1347
  5. Engler A. and Wang Y. (2021) Understanding Molecular Interactions that Underpin Vascular Mechanobiology, APL Bioengineering, 5(3):030401
  6. Peng Q., Weng K., Li S., Xu R., Wang Y. and Wu Y. (2021) A Perspective of Epigenetic Regulation in Radiotherapy, Frontiers in Cell and Developmental Biology, 9:624312
  7. Li S.-S., Gao S., Chen Y., Bao H., Li Z.-T., Yao Q.-P., Liu J.-T., Wang Y. and Qi Y.-X. (2021) Platelet-derived microvesicles induce calcium oscillations and promote VSMC migration via TRPV4, Theranostics, 11(5):2410-2423
  8. Harrison, R., Weng K., Wang Y., and Peng Q. (2021) Phase Separation and Histone Epigenetics in Genome Regulation, Current Opinion in Solid State and Materials Science, 25(1):100892


  1. Liu L., He F., Yu Y. and Wang Y. (2020) Application of FRET Biosensors in Mechanobiology and Mechanopharmacological Screening, Frontiers in Bioengineering and Biotechnology, 8:595497
  2. Zhuang F., Bao H., Shi Q., Wang W.B., Li J., Jiang Z.L., Wang Y., Qi Y. (2020) Endothelial Microparticles Induced by Cyclic Stretch Activate Src and Modulate Cell Apoptosis, FASEB Journal, 34(10):13586-13596
  3. Yoon S., Pan Y., Shung K., Wang Y. (2020) High frequency ultrasound based single cell resolution FRET Ca2+ biosensor imaging, Sensors, 20(17):4998
  4. Zhu L., Wu Y., Yoon C., and Wang Y. (2020) Mechanogenetics for Cellular Engineering and Cancer Immunotherapy, Current Opinion in Biotechnology, 66:88-94
  5. Liu L., Yu H., Zhao H., Wu Z., Long Y., Zhang J., Yan X., You Z., Zhou L., Xia T., Shi Y., Xiao B., Wang Y., Huang C., Du Y. (2020) Matrix-transmitted paratensile signaling enables myofibroblast-fibroblast crosstalk in fibrosis expansion, Proc Natl Acad Sci USA, pii: 201910650
  6. Huang Z., Ouyang M., Lu S., Wang Y., and Peng Q. (2020) Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells, Journal of Molecular Biology, 432(7):1901-1909
  7. Huang N.F., Chaudhuri, O., Cahan, P., Engler A.J., Wang Y., Kumar S., Khademhosseini A., Li S. (2020) Multi-Scale Cellular Engineering: From Molecules to Organ-on-a-Chip, APL Bioengineering, 4(1):010906
  8. Li S., Yang D., Gao L., Wang Y., and Peng Q. (2020) Epigenetic Regulation and Mechanobiology, Biophysics Reports, Accepted,
  9. Dutta P., Zhang L., Zhang H., Peng Q., Montgrain P.R., Wang Y., Song Y., Li J., Li W.X. (2020) Unphosphorylated STAT3 in heterochromatin formation and tumor suppression in lung cancer, BMC Cancer, 20(1):145,
  10. Huang Z., Wu Y., Allen M., Pan Y., Kyriakakis P., Lu S., Chang Y.-J., Wang X., Chien S., and Wang Y. (2020) Engineering Light-controllable CAR T Cells for Cancer Immunotherapy, Science Advances, 6(8):eaay9209
  11. Lu S., Qin Q., Laub S., Shi Y., Ouyang M., Peng Q., Zhang J., Wang Y. (2020) Fluocell for Ratiometric and High-throughput Live-cell Image Visualization and Quantitation, Frontiers in Physics, section Computational Physics, 7:154


  1. Zamat A., Zhu L., and Wang Y. (2019) Engineering Molecular Machines for the Control of Cellular Functions for Diagnostics and Therapeutics, Advanced Functional Materials, 1904345,
  2. Allen M.E., Zhou W., Thangaraj J., Kyriakakis P., Wu Y., Huang Z., Ho P., Pan Y., Limsakul P., Xu X., and Wang Y. (2019) An AND-gated Drug and Photoactivatable Cre-loxP System for Spatiotemporal Control in Cell-based Therapeutics, ACS Synthetic Biology, 8(10):2359-2371,
  3. Vennin C., Melenec P., Romain R., Nobis M., Cazet A.S., Murphy K.J., Herrmann D., Reed D.A., Lucas M.C., Warren S.C., Elgundi Z., Pinese M., Kalna G., Roden D., Samuel M., Zaratzian A., Grey S., Silva A.D., Leung W., Mathivanan S., Wang Y., Braithwaite A.W., Christ D., Benda A., Parkin A., Phillips P.A., Whitelock J.M., Gill A.J., Sansom O.J., Croucher D.R., Parker B.L., Pajic M., Morton J.P., Cox T.R., Timpson P. (2019) CAF hierarchy driven by pancreatic cancer cell p53-status creates a pro-metastatic and chemoresistant environment via perlecan, Nature Communications, 10(1):3637
  4. Wan R., Wu J., Ouyang M., Lei L., Wei J., Peng Q., Harrison R., Wu Y., Cheng B., Li K., Zhu C., Tang L., Lu S., Wang Y. (2019) Biophysical Basis underlying Dynamic Lck Activation Visualized by ZapLck FRET Biosensor, Science Advances, 5(6):eaau2001, (Data)
  5. Xie SA., Yao W., Wang Y., Tang RZ., Zhu JJ., Liu YF., Han LL., Pang W., Yang FF., Zhang T., Zhang YP., Kong W., and Zhou J. (2019) DNA Methyltransferase 1 and Krüppel-like Factor 4 Axis Regulates Macrophage Inflammation and Atherosclerosis, Journal of Molecular and Cellular Cardiology, 128:11-24
  6. Wu Y. and Wang Y. (2019) Protein Circuits Reprogram Cells, Nature Chemical Biology, 15(2):96-97
  7. Pan Y., Lu S., Lei L., Lamberto I., Wang Y., Pasquale E., Wang Y. (2019) A genetically encoded FRET biosensor for visualizing EphA4 activity in different compartments of the plasma membrane, ACS Sensors, 4(2):294-300
  8. Ouyang M., Wan R., Qin Q., Peng Q., Wang P., Wu J., Allen M., Shi Y., Laub S., Deng L., Lu S., Wang Y. (2019) A sensitive FRET biosensor reveals Fyn kinase regulation by sub-membrane localization, ACS Sensors, 4(1):76-86


  1. Kim, TJ., Lei L., Seong J., Suh JS., Jang YK., Jung SH., Sun J., Kim DH., and Wang Y. (2018) Matrix rigidity-dependent regulation of Ca2+ at plasma membrane microdomains by FAK visualized by fluorescence resonance energy transfer, Advanced Science, 6(4):1801290
  2. Peng Q., Lu S., Shi Yx.., Pan Y., Linsakul P., Shi Yw., Chernov AV., Qiu J., Chai X., Wang P., Ji Y., Li Y.-S., Strongin AY., Verkhusha VV., Belmonte JCI, Ren B., Wang Y.L., Chien S., and Wang Y. (2018) Coordinated Histone Modifications and Chromatin Reorganization in A Single Cell Revealed by FRET Biosensors, Proc Natl Acad Sci USA, 115(50):E11681-E11690
  3. Pan Y., Zhu L., Yoon S., and Wang Y. (2018) Acoustic Mechanogenetics, Current Opinion in Biomedical Engineering, DOI: 10.1016/j.cobme.2018.10.001 7:64-70
  4. Hard R., Li N., He W., Ross B., Mo GCH, Peng Q., Stein R., Komives E., Wang Y., Zhang J., and Wang W. (2018) Deciphering and Engineering Chromodomain-Methyllysine Peptide Recognition, Science Advances, 4(11):eaau1447
  5. Wang P., Liang J., Shi L., Wang Y., Zhang P., Ouyang M., Preece D., Peng Q., Shao L., Fan J., Sun J., Li S., Berns M., Zhao H., Wang, Y. (2018) Visualizing spatiotemporal dynamics of intercellular mechanotransmission upon wounding, ACS Photonics, 5(9):3565-3574
  6. Tang X., Liu Y., Xiao Q., Yao Q., Allen M., Wang Y., Gao L., Qi Y., Zhang P. (2018) Pathological cyclic strain promotes proliferation of vascular smooth muscle cells via the ACTH/ERK/STAT3 pathway, J Cell Biochem, DOI: 10.1002/jcb.26839 119(10):8260-8270
  7. Warren SC, Nobis M, Magenau A, Mohammed YH, Herrmann D, Moran I, Vennin C, Conway JRW, Mélénec P., Wang Y., Morton JP., Phan T., Roberts MS and Timpson P. (2018) Removing physiological motion from intravital and clinical functional imaging data, eLife, 7. pii: e35800. doi: 10.7554/eLife.35800
  8. Conway JRW, Warren SC, Herrmann D, Murphy KJ, Cazet AS, Vennin C, Shearer RF, Killen MJ, Magenau A, Mélénec P, Pinese M, Nobis M, Zaratzian A, Boulghourjian A, Da Silva AM, Del Monte-Nieto G, Adam ASA, Harvey RP, Haigh JJ, Wang Y, Croucher DR, Sansom OJ, Pajic M, Caldon CE, Morton JP, Timpson P. (2018) Intravital Imaging to Monitor Therapeutic Response in Moving Hypoxic Regions Resistant to PI3K Pathway Targeting in Pancreatic Cancer, Cell Reports. 23(11):3312-3326.
  9. Randolph E. Bank, Yiqian Wu, Yiwen Shi, Philip E. Gill, Yingxiao Wang, Shaoying Lu, Optimization with Partial Differential Equation Constraints for Bio-imaging Applications, Computing and Visualization in Science (Accepted)
  10. Pan Y., Yoon S., Sun J., Huang Z., Lee C., Allen M., Wu Y., Chang Y.-J., Sadelain M., Shung K.K., Chien S., and Wang Y. (2018) Mechanogenetics for the remote and noninvasive control of cancer immunotherapy, Proc Natl Acad Sci USA, 115(5):992-997
  11. Limsakul P., Peng Q., Wu Y., Allen M., Liang L., Remacle AG., Lopez T., Ge X., Kay B., Zhao H., Strongin AY., Yang Y.-L., Lu S., and Wang Y. (2018) Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface, Cell Chemical Biology, 25(4):370-379.e4.


  1. Wan Q., TruongVo T., Steele H.E., Ozcelikkale A., Han B., Wang Y., Yokota H., Oh J., Na S. (2017) Subcellular domain-dependent molecular hierarchy of SFK and FAK in mechanotransduction and cytokine signaling, (Nature) Scientific Reports, 10.1038/s41598-017-09495-5.
  2. Sun J., Lei L. Tsai C.-M., Wang Y., Shi Y., Ouyang M., Lu S., Seong J., Kim T., Wang P., Huang M., Xu X., Nizet V., Chien, S., Wang Y. (2017) Engineered Proteins with Sensing and Activating Modules for Automated Reprogramming of Cellular Functions, Nature Communications, 10.1038/s41467-017-00569-6.
  3. Yoon S., Wang P., Peng Q., Wang Y., Shung K.K. (2017) Acoustic-transfection for genomic manipulation of single-cells using high frequency ultrasound, (Nature) Scientific Reports, 10.1038/s41598-017-05722-1.
  4. Seong J., Huang M., Sim K., Kim H., Wang Y. (2017) FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains, (Nature) Scientific Reports, 10.1038/s41598-017-01789-y.
  5. Vennin C., Chin V.T., Warren S.C., Lucas M.C., ... Wang Y., ... Pajic M., Timpson P. (2017) Transient tissue priming via ROCK inhibition uncouples pancreatic cancer progression, sensitivity to chemotherapy and the onset of the metastatic niche, Science Translational Medicine, 10.1126/scitranslmed.aai8504.


  1. Mao M., Wang L., Chang C.-C., Rothenberg K., Huang J., Wang Y., Hoffman B., Liton P., and Yuan F. (2016) Involvement of a Rac1-dependent macropinocytosis pathway in plasmid DNA delivery by electrotransfection, Molecular Therapy, 25(3): 803-815.
  2. Wang Y. (2016) FRET imaging of molecular hierarchy at subcellular levels in mechanotransduction, Journal of Medical Biomechanics, accepted.
  3. Li K., Xiang X., Sun J., He H.-T., Wu J., Wang Y., and Zhu C. (2016) Imaging Spatiotemporal Activities of ZAP-70 in Live T Cells Using a FRET-based Biosensor, The Annals of Biomedical Engineering, 44(12): 3510-3521.
  4. Wu Y., Zhang K., Chien S., Wang Y., and Lu S. (2016) In-situ coupling between kinase activities and protein dynamics within single focal adhesions. (Nature) Scientific Reports, 6: 29377.
  5. Qi Y.X., Yao Q.P., Huang K., Shi Q., Zhang P., Wang G.L., Han Y., Bao H., Wang L., Li H.P., Shen B.H., Wang Y., Chien S., Jiang Z.L. (2016) Nuclear Envelope Proteins Modulate Proliferation of Vascular Smooth Muscle Cells during Cyclic Stretch Application. Proc Natl Acad Sci USA, 113(19): 5293-5298.
  6. Peng Q., Cheng B., Lu S., Chien S., and Wang Y. (2016) Perspectives of FRET Imaging to study Epigenetics and Mechanobiology in the Nucleus, Molecular and Cellular Mechanobiology, Springer.
  7. Wang Y. (2016) New Technologies of Molecular Engineering and Screening for Cell Signaling Studies. Molecular Biology of the Cell, 27(6): 875-876.
  8. Le Borgne M., Raju S., Zinselmeyer BH., Le VT., Li J., Wang Y., Miller MJ., Shaw AS. (2016) Real-Time Analysis of Calcium Signals during the Early Phase of T Cell Activation Using a Genetically Encoded Calcium Biosensor . The Journal of Immunology 196(4): 1471-9.
  9. Yoon S., Kim MG., Chiu CT., Hwang JY., Kim HH., Wang Y., and Shung K. (2016) Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound, (Nature) Scientific Reports 6: 20477
  10. Wang R, Lei L, Sridharan S, Wang Y, Levine AJ, Popescu G. (2016) Dispersion relations of cytoskeleton dynamics, Cell Health and Cytoskeleton 8: 1-7.


  1. Wang X., Sun J., Xu Q., Chowdhury F., Roein-Peikar M., Wang Y., and Ha T. (2015) Integrin Molecular Tension within Motile Focal Adhesions, Biophysical Journal 109(11): 2259-2267.
  2. Chung E., Ochs C.J., Wang Y., Lei L., Qin Q., Smith A.M., Strongin A.Y., Kamm R., Qi Y.-X., Lu S., Wang Y. (2015) Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells, Nano Letters 15(8): 5025-5032.
  3. Zhuo Y., Qian T., Wu Y., Seong J., Gong Y., Ma H., Wang Y., Lu S. (2015) Subcellular and Dynamic Coordination between Src Activity and Cell Protrusion under Microenvironment modulations, (Nature) Scientific Reports 5:12963.
  4. Lee K.L., Guevarra M.D., Nguyen A.M., Chua M.C., Ouyang M., Wang Y., Jacobs J.R. (2015) The primary cilium functions as a mechanical and calcium signaling nexus, Cilia 4:7.
  5. Nishitani1 W.S., Alencar A.M., Wang Y. (2015) Rapid and localized mechanical stimulation and adhesion assay: TRPM7 involvement in calcium signaling and cell adhesion, PLoS ONE 10(5):e0126440.
  6. Han Y., Wang L., Yao Q., Zhang P., Liu B., Wang G.-L., Shen B., Cheng B., Wang Y., Jiang Z., Qi Y. (2015) Nuclear Envelope Proteins Nesprin2 and LaminA Regulate Proliferation and Apoptosis of Vascular Endothelial Cells in Response to Shear Stress, BBA - Molecular Cell Research 1853(5):1165-73.
  7. Kim T.J., Joo C., Seong J., Vafabakhsh R., Botvinick E.L., Berns M.W., Palmer A.E., Wang N., Ha T., Jakobsson E., Sun J., Wang Y. (2015) Distinct mechanisms regulating mechanical force-induced Ca2+ signals at the plasma membrane and the endoplasmic reticulum in human mesenchymal stem cells, eLife 4:e04876.
  8. Sun J., Jakobsson E., Wang Y., Brinker J. (2015) Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine, Life, 5(1):214-229
  9. Gomez-Godinez V., Shi L.Z., Khatibzadeh N., Rosales D., Preece D., Lei L., Wang Y., Berns M.W. (2015) Laser Induced Shockwave Paired with FRET to Study Intracellular Calcium Release, Microscopy Research and Technique 78(3):195-9.
  10. Chung E.Y., Qin Q., Bhattacharyya A., Lu S., Wang Y. (2015) Nanotechnologies and FRET imaging in live cells, Micro and Nano Techniques in Cell Mechanobiology, Cambridge University Press, In Press
  11. Kim T.J., Zheng S., Sun J., Muhamed I., Wu J., Lei L., Kong X., Leckband D.E., and Wang Y. (2015) Dynamic visualization of a-catenin reveals rapid, reversible conformation switching between tension states, Current Biology, 25(2):218-24


  1. Liu B., Lu S., Hu Y.-L, Liao X., Ouyang M., Wang Y. (2014) RhoA and Membrane Fluidity Mediates the Spatially Polarized Src/FAK Activation in response to Shear Stress, (Nature) Scientific Reports, 4:7008
  2. Hwang K., Wu P., Kim T.J., Lei L., Tian S., Wang Y., Lu Y. (2014) Photocaged DNAzymes as a General Method for Sensing Metal Ions in Living Cells, Angewandte Chemie International Edition 53(50):13798-802
  3. Kim T.J., Sun J., Lu S., Qi Y.X., Wang Y. (2014) Prolonged mechanical stretch initiates intracellular calcium oscillations in human mesenchymal stem cells, PLoS ONE, 9(10):e109378
  4. Sun C., Ouyang M., Alqublan H., Sriranganathan N., Wang Y. and Lu C. (2014) Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding, Chem. Comm. 50(78):11536-9
  5. Lu S. and Wang Y. (2014) Single-Cell Imaging of Mechanotransduction in Endothelial Cells. Progress in Molecular Biology and Translational Science. In Press
  6. Aung A., Seo T., Lu S., Wang Y., Jamora C., del Alamo JC, and Varghese S. (2014) 3D Traction stresses activate protease-dependent invasion of cancer cells, Biophysical Journal, 107(11):2528-37
  7. Tan Y., Tajik A., Chen J., Jia Q., Chowdhury F., Wang L, Chen J., Zhang S., Hong Y., Yi H., Wu D., Zhang Y., Wei F., Poh Y.-C., Singh R., Seong J., Lin L.-J., Doganay S., Li Y., Jia H., Ha T., Wang Y., Huang B., Wang N. (2014) Matrix softness regulates plasticity of tumor-repopulating cells via H3K9 demethylation and Sox2 expression, Nature Communications, 5:4619
  8. Hu Y.-L., Lu S., Szeto K.W., Sun J., Wang Y., Lasheras J.C., Chien S. (2014) FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells, (Nature) Scientific Reports, 4:6024
  9. Lu S., Seong J., Chang S.C., Wang Y., Eichorst J.P., Holzer R.G., Ouyang M., Chien S., and Wang Y. (2014) Decipher the dynamic coordination between enzymatic activity and structural modulation at focal adhesions in living cells, (Nature) Scientific Reports, 4:5756
  10. Kim TJ, Sun J, Lu S, Zhang J, Wang Y. (2014) The regulation of beta-adrenergic receptor-mediated PKA activation by substrate stiffness through microtubules in human mesenchymal stem cells, Biomaterials, 35(29):8348-56
  11. Hum JM., Day RN., Bidwell JP., Wang Y. Pavalko FM (2014) Mechanical Loading in Osteocytes Induces Formation of a Src/Pyk2/MBD2 Complex That Suppresses Anabolic Gene Expression, PLoS ONE, 9(5):e97942
  12. Lei L., Lu S., Wang Y., Wang Y. (2014) The role of mechanical tension on lipid rafts dependent PDGF-induced TRPC6 activation, Biomaterials, 35(9):2868-77
  13. Ouyang M., Lu S., Wang Y. (2014) Genetically Encoded Fluorescent Biosensors for Live-cell Imaging of MT1-MMP Protease Activity, Methods in Molecular Biology, 1071:163-74


  1. Ntziachristos V., Achilefu S., Wang Y., Lin M. (2013). Special Section on Fluorescence Molecular Imaging Honoring Prof. Roger Tsien, a Pioneer in Biomedical Optics. J Biomed Opt. 18(10):10130
  2. Seong J., Tajik A., Sun J., Guan J.-L., Humphries M.J., Craig S.E., Shekaran A., Garcia A.J., Lu S., Lin M.Z., Wang N., Wang Y. (2013) Distinct biophysical mechanisms of FAK mechanoactivation by different extracellular matrix proteins. Proc Natl Acad Sci USA (Direct Submission), 110(48):19372-7
  3. Liao X., Lu S., Zhuo Y., Wang Y. (2013) The effect of inducible reagent on Src and FAK activity during MSCs differentiation. PLoS ONE, 8(8):e72233
  4. Nobis M, McGhee EJ, Morton JP, Schwarz JP, Karim SA, Quinn J, Edward M, Campbell AD, McGarry LC, Evans TR, Brunton VG, Frame MC, Carragher NO, Wang Y, Sansom OJ, Timpson P, Anderson KI. (2013) Intravital FLIM-FRET imaging reveals dasatinib induced spatial control of Src in pancreatic cancer, Cancer Research, 73(15):4674-86
  5. Sun J., Lu S., Ouyang M., Lin L.J., Zhuo Y., Liu B., Chan R., Chien S., Neel B.G., Wang Y. (2013) Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2, Nature Communications, 4:2037
  6. Lu S., Huang H., Wang Y., Strongin AY., Wang Y. (2013) Highly Sensitive FRET Biosensors for Detecting MMP Activity in Live Breast Cancer Cells, PLoS ONE, 8(3): e58569
  7. Seong J., Wang N., Wang Y. (2013) Mechanotransduction at Focal Adhesions: from Physiology to Cancer Development, Journal of Cellular and Molecular Medicine, doi: 10.1111/jcmm.12045. [Epub ahead of print]
  8. Ouyang M., Lu S., Chen CN., Kim T., Seong J., Leckband DE., Wang F., Reynolds AB., Schwartz MA., Wang Y. (2013) N-cadherin Differentially Couples with p120ctn and b-catenin to Regulate the Polarity of Distinct Molecular Activities, Nature Communications, 4:1589
  9. Lin L.-J., Grimme J.M., Sun J., Lu S., Gai L., Cropek D.M., Wang Y. (2013) The antagonistic roles of PDGF and integrin αγβ3 in regulating ROS production at focal adhesions, Biomaterials, 34(15):3807-15
  10. Qian T., Lu S., Ma H., Fang J., Zhong W., Wang Y. (2013) FRET Imaging of Microenvironment Effects on Calcium Signaling in Live Cells, Integrative Biology, 5(2):431-8


  1. Brown G, Butler PJ, Chang DW, Chien S, Clegg RM, Dewey CF, Dong C, Guo XE, Helmke BP, Hess H, Jacobs CR, Kaunas RR, Kumar S, Lu HH, Mathur AB, Mow VC, Schmid-Schönbein GW, Skoracki R, Wang N, Wang Y, Zhu C. (2012) Cellular and Molecular Bioengineering: A Tipping Point, Cellular and Molecular Bioengineering, 5(3):239-253
  2. Wang R., Lei L., Wang Y., Levine A.J., and Popescu G. (2012) Dispersion-relation fluorescence spectroscopy, Physics Review Letter, 109(18):188104
  3. *Liao X., *Lu S., Zhuo Y., Wang Y. (2012) Visualization of Src and FAK activity during the differentiation process from HMSCs to osteoblast by FRET biosensor, PLoS ONE, 7(8):e42709
  4. Eichorst JP., Clegg RM., Wang Y. (2012), Red-Shifted Fluorescent Proteins Monitor Enzymatic Activity in Live HT-1080 Cells with Fluorescence Lifetime Imaging Microscopy (FLIM), Journal of Microscopy, 248(1):77-89
  5. Birukova A.A., Tian Y., Dubrovskyi O., Zebda N., Sarich N., Tian X., Wang Y., Birukov K.G. (2012) VE-cadherin Trans-interactions Modulate Rac Activation and Enhancement of Lung Endothelial Barrier by Iloprost, Journal of Cellular Physiology, 227(10):3405-16


  1. *Xiang X., *Sun J., Wu J., He H.T., Wang Y., Zhu C. (2011) A FRET-based biosensor for imaging SYK activities in living cells, Cellular and Molecular Bioengineering, 4(4):670-677
  2. Nishitani WS., Saif TA, Wang Y. (2011) Calcium signaling in live cells on elastic gels under mechanical vibration at subcellular levels, PLoS ONE, 6(10):e26181
  3. *Liao X., *Lu S., Zhuo Y., Winter C., Xu W., Li B., Wang Y. (2011) Bone physiology, biomaterial and the Effect of mechanical/physical microenvironment onmesenchymal stem cell osteogenesis, Cellular and Molecular Bioengineering, 6(6):579-590
  4. Seong J., Ouyang M., Kim T., Sun J., Wen P.-C., Lu S., Zhuo Y., Llewellyn N.M., Schlaepfer D.D., Guan J.L., Chien S., Wang Y. (2011) Detection of focal adhesion kinase activation at Membrane Microdomains by fluorescence resonance energy transfer, Nature Communications, 2:406
  5. Lu S., Kim T., Chen CN., Ouyang M., Seong J., Wang Y. (2011) Computational Analysis of the Spatiotemporal Coordination of Molecular Activities in Live Cells, PLoS ONE, 6(6):e21293
  6. Eichorst JP., Huang H., Clegg RM., Wang Y. (2011) Phase differential enhancement to distinguish fluorescence components when monitoring molecular activity in live cells by FLIM, Journal of Fluorescence, 21(4):1763-77
  7. Liu B., Lu S., Zheng S., Jiang Z.-L., Wang Y. (2011) Two Distinct Phases of Calcium Signaling under Flow, Cardiovascular Research, 91(1):124-33
  8. Seong J., Lu S., Wang, Y. (2011) Live Cell Imaging of Molecular Signaling by FRET, Cellular and Molecular Bioengineering, 4(2):138-147
  9. Butler P.J., Wang, Y. (2011) Editorial Note: Molecular Imaging and Mechanobiology, Cellular and Molecular Bioengineering, 4(2):123-124
  10. Asada HH., Wang Y., Mayalu MN. (2011) Molecular Signaling Observer and Predictor: A Framework for Closed-Loop Control of Cell Behaviors Having Long Time Delay, American Control Conference. 1240:2314-2319


  1. Lu, S., Wang, Y. (2010) FRET Biosensors for Cancer Detection and Evaluation of Drug Efficacy, Clinical Cancer Research, 16(15):3822-4
  2. Gawlik K., Remacle AG., Shiryaev SA., Golubkov VS., Ouyang M., Wang Y., and Strongin AY. (2010) A femtomol range FRET biosensor reports exceedingly low levels of cell surface furin: implications for the processing of anthrax protective antigen, PLoS ONE, 5(6):e11305
  3. Qian T., Wang Y. (2010) Micro/nano-fabrication Technologies for Cell Biology. Medical & Biological Engineering & Computing, 48(10):1023-32
  4. Liu B., Kim T., Wang Y. (2010) Live Cell Imaging of Mechanotransduction. Journal of the Royal Society Interface, 7 Suppl 3:S365-75
  5. Ouyang M.*, Huang H.*, Shaner N., Remacle AG., Shiryaev SA., Strongin AY., Tsien RY., Wang Y. (2010) Simultaneous Visualization of Pro-tumorigenic Src and MT1-MMP Activities with Fluorescence Resonance Energy Transfer. Cancer Research, 70(6):2204-12


  1. Poh Y.C., Na S., Chowdhury F., Ouyang M., Wang Y., Wang N. (2009) Rapid Activation of Rac GTPase in Living Cells by Force Is Independent of Src. PLoS ONE,4(11):e7886
  2. Wang Y., Wang N. (2009) FRET and Mechanobiology. Integrative Biology, 1(10):565-73
  3. Wang Y.*, Flores L.*, Lu S., Miao H., Li Y.-S., Chien S. (2009) Shear Stress Regulates the Flk-1/Cbl/PI3K/NF-kB Pathway via Actin and Tyrosine Kinases. Cellular and Molecular Bioengineering, 2(3):341-350
  4. Kim T., Wang Y. (2009) Single cell imaging of calcium dynamics in response to mechanical stimulation. Cell Mechanics: From Single Scale-Based Models to Multiscale Modeling, 10:265-279
  5. Lu S., Wang Y. (2009) Application of FRET Biosensors and Computational Analysis for Live Cell Imaging. Proc. of SPIE. 7191 (719108):1-12
  6. Kim T., Xu J., Dong R., Lu S., Nuzzo R., Wang Y. (2009) Visualizing the Effect of Microenvironment on the Spatiotemporal RhoA and Src Activities in Live Cells by FRET. Small, 5(12):1453-9.
  7. Na S., Chowdhury F., Tay B., Ouyang M., Gregor M., Wang Y., Wiche G., Wang N. (2009) Plectin contributes to mechanical properties of living cells. Am J PhysiolCell Physiol, 296(4):C868-77
  8. Seong J., Lu, S., Ouyang M., Huang H., Zhang J., Frame MC., Wang Y. (2009) Visualization of Src activity at different compartments of the plasma membrane by FRET imaging. Chemistry & Biology (Cell Press), 16(1):48-57, (Cover Article)
  9. Kim T., Seong J., Ouyang M., Sun, J., Lu, S., Hong J., Wang, N., Wang Y. (2009) Mechanical rigidity regulates Ca2+ oscillation via RhoA signaling pathway in humanmesenchymal stem cells. Journal of Cellular Physiology, 218(2):285-93


  1. *Eichorst J., *Lu S., Xu J., Wang, Y. (2008) Differential RhoA Dynamics in Migratory and Stationary Cells Measured by FRET and Automated Image Analysis. PLoS ONE, 3(12):e4082
  2. Ouyang M., Sun J., Chien S., Wang Y. (2008) Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors, Proc Natl Acad Sci USA. 105(38):14353-8
  3. Wang, Y., Shyy Y.-J., Chien, S. (2008) Fluorescence Proteins, Live Cell Imaging, and Mechanobiology: Seeing is Believing, Annual Review of Biomedical Engineering, 10:1-38 (The most downloaded article of ARBE in year 2009)
  4. Lu S., Ouyang M., Seong J., Zhang J., Chien, S., Wang Y. (2008) The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging. PLoS Computational Biology, 4(7): e1000127, (Featured Research by PLoS Computational Biology)
  5. Na S., Collin O., Chowdery F., Tay B., Ouyang M., Wang Y., Wang N. (2008) Rapid signal transduction in living cells is a unique feature of mechanotransduction. ProcNatl Acad Sci USA. 105 (18):6626-31
  6. Ouyang M., Lu S., Li X.-Y., Xu J., Seong J., Giepmans B., Shyy Y.-J., Weiss SJ, Wang Y. (2008) Directional MT1-MMP Activity in Live Cells Visualized by a FRET Biosensor, Journal of Biological Chemistry. 283 (25):17740-8
  7. Wang Y., Lu S. (2008) The Application of FRET Biosensors to Visualize Src Activation. Proc. of SPIE. 6868 (68680A):1-9
  8. Wang Y., Shyy, J.-Y., and Chien, S., (2008) Fluorescence Live-Cell Imaging: Principles and Applications in Mechanobiology. Bioengineering in Cell and Tissue Research, pp. 65-84


  1. Wang Y. and Chien, S., (2007) Analysis of Integrin Signaling by Fluorescence Resonance Energy Transfer. Methods in Enzymology, 426:177-201
  2. Botvinick, EL and Wang Y., (2007) Laser Tweezers in the Study of Mechanobiology in Live Cells. Methods in Cell Biology, 82:497-523
  3. Wang Y., Chang JM., Chen K.-D., Li S., Li J., Wu C., Chien S. (2007) Selective Adapter Recruitment and Differential Signaling Networks by VEGF vs. Shear Stress. Proc Natl Acad Sci USA. 104 (21): 8875-9
  4. Larina IV., Wang Y., Chien S., Lane ME., and Dickinson ME. (2007) On the way to subcellular imaging of mechanotransduction in the developing vasculature. Proc. of SPIE. 6535 (653504):1-6


  1. Hu YL., Haga JH., Miao H., Wang Y., Li YS., Chien S. (2006) Roles of microfilaments and microtubules in paxillin dynamics. Biochem Biophys Res Commun. 348(4):1463-71.
  2. Wang Y., Jin G., Miao H., Li J., Usami S., Chien S. (2006) Integrins Regulate VE-cadherin and Catenins: Dependence of This Regulation on Src, but not on Ras. Proc Natl Acad Sci USA. 103(6):1774-79.


  1. Wang Y., Botvinick E., Zhao Y., Berns M., Usami S., Tsien R.Y., Chien S. (2005) Visualization of Mechanical Activation of Src. Nature. 434:1040.
  2. Miao H., Hu Y., Shiu Y.-T., Yuan S., Zhao Y., Kaunas R., Wang Y., Jin G., Usami S., and Chien S. (2005) Effects of Flow Patterns on the Localization and Expression of VE-Cadherin at Vascular Endothelial Cell Junctions: In-vivo and In-vitro Investigations. Journal of Vascular Research. 42:77.
  3. Wang Y., Chang JM., Li Y.-C., Li Y.-S., Shyy J. Y.-J., and Chien S. (2004) Shear Stress and VEGF Activate IKK via Flk-1/Cbl/Akt Signaling Pathway. Am J PhysiolHeart Circ Physiol. 286(2):H685-92.
  4. Shiu Y.-T., Li S., Yuan S., Wang Y., Nguyen P., Chien S. (2003) Shear stress-induced c-fos activation is mediated by Rho in a calcium-dependent manner. BiochemBiophys Res Commun. 303(2):548-55
  5. Wang Y., Miao H., Li S., Chen K.-D., Li Y.-S., Yuan S., Shyy J. Y.-J., and Chien S., (2002) Interplay between integrins and FLK-1 in shear stress- induced signaling. Am J Physiol Cell Physiol. 283: C1540-1548
  6. Miao H., Yuan S., Wang Y., Tsygankov A., Chien S. (2002) Role of Cbl in Shear-Activation of PI 3-kinase and JNK in Endothelial Cells. Biochem Biophys ResCommun. 292(4):892-9
  7. Li S., Butler P., Wang Y., Hu Y., Han D., Usami S., Guan J.-L., Chien S. (2002). The role of the dynamic of focal adhesion kinase in the mechanotaxis of endothelial cells. Proc Natl Acad Sci USA. 99(6):3546-51
  8. Wu W., Wang Y., Yan Z., Wen G. (1999). The Dynamic Coupling Model for the Whole Circulation in Human Body. Acta S cientiarum Naturalium, Universitatis Pekinensis. 35(3): 328-339
  9. Wu W., Wen G., Yan Z., Wang Y., Zhang, Z. (1999). The Dynamic Coupling of Right Ventricle and Pulmonary Arteries. Acta Scientiarum Naturalium, Universitatis Pekinensis. 35(3): 317-327
  10. Wang Y., Wu W., Wen G. (1999). The Influence of Aortic Valve on the Cardiovascular Coupling. Journal of Hydrodynamics. 14(1): 13-23.
  11. Wang Y., Wu W., Wen G. (1998). The Influence of Cardiovascular Dynamic Coupling on the Blood Pulse Wave Propagation in Human Body. Applied Mathematics and Mechanics. 19(7):601-614

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