Smiley face Molecular Engineering for Cellular Imaging and Reprogramming

1. Competent Cell Preparation

♦ Competent Cell Preparation

♦ Electro-competent

2. Transformation

♦ Transformation Procedure

3. Amplification

a. Miniprep

DNA Amplification Mini Prep

b. Maxiprep

Detailed_Maxi_Prep_Procedure

DNA Amplification Maxi Prep

♦ Measure DNA Concentration

♦ Shipping and Receiving Plasmids on Filter Paper

4. Primer Design

♦ Primer design concerns

5. PCR and Cloning Strategies

♦ Colony_PCR

♦ Deletion and Addition Inserts

♦ Multiple mutations

♦ PCR Programs

♦ PCR_reaction_protocol

♦ Standard PCR

6. Digestion

♦ DNA Digestion

7. Gel Purification

8. Ligation

♦ Ligation of reporter to Adenovirus Ad5

♦ Ligation Samples

9. Miscellaneous

♦ general Molecular cloning Protocols

 

Copyright: Peter Wang Lab. WebMaster - kalu@ucsd.edu , qqin@eng.ucsd.edu