Smiley face Molecular Engineering for Cellular Imaging and Reprogramming

1. Microscope start up and shut down procedures

♦ Confocal Microscopy Usage

♦ Rules and Tips for the Microscope Operation

♦ Starting Protocol for Nikon Microscope

♦ Startup_Protocol_for_Zeiss_scope

2. Microscope Optical Setting and Diagrams

♦ DIC microscope setting

♦ Nikon Microscope Parameters

♦ Zeiss Microscope Parameters

3. Live Cell Imaging

♦ Agarose Gel Protocol

♦ Cell_Imaging_Preparation_General_Procedure

♦ EDTA Solution

♦ EGF Experiment

♦ FRAP experiment protocol

♦ FRET related techniques

♦ Protocol of preparing adhesion assay imaging

4. Miscellaneous

♦ Alignment of Microscopy

♦ Conversions

♦ Kohler Alignment for Nikon

♦ microscope_cartoon(pptx)

♦ microscope_cartoon(pdf)

5. Nikon

♦ 20130926 photoactivation Microscope settings-1

♦ illumination_control_1213_2013

♦ NikonMicroscopeParameters

♦ NikonMicroscopeParameters_old

♦ 20130926 photoactivation Microscope settings-1

♦ illumination_control_1213_2013

♦ Nikon measurement and adjustment 2013Oct9

♦ nikon_neutrodensity_shutter(figure)

♦ nikon_neutrodensity_shutter(pptx)

♦ NK92_cell_culture

♦ NK92_cell_transfection

 

Copyright: Peter Wang Lab. WebMaster - kalu@ucsd.edu , qqin@eng.ucsd.edu