Smiley face Molecular Engineering for Cellular Imaging and Reprogramming

1. Transfection

♦ Lipofectimine 2000

♦ Transfection Proteins Ras and Src Treatment

2. Immunoprecipitation and Immunoblotting

♦ Immunoprecipitation and Immunoblotting

3. Immunostaining

biotin_avidin_blocking

Immunostaining General Protocol

Immunostaining of Biotin-Streptavidin System

Staining_biotin

4. Constructs

♦ c-Src Mutations

♦ Hamster c-ha-ras cds

♦ Membrane_binding_domain_review

♦ PH_domain_GFPs

♦ PIPs_Demirovic

5. Miscellaneous

♦ Antibodies

♦ Clustering of Epherin

♦ Coat Beads with Fibronectin

♦ CollagenConjugateRhodamin

♦ Inhibitors

♦ Integrin Antibodies

♦ Lysis Buffer

♦ Pervanadate

♦ Protocols for coupling carboxyl beads to EGF or Fibronectin

♦ Purify Protein Invitro Kinase Assay

♦ RGD Peptide Inhibitions

♦ Stimulation of Integrins

♦ Coupling_protein_carboxyl_beads

♦ Proteasephosphatase

♦ FPLC protocol

♦ His tag protein purification

 

Copyright: Peter Wang Lab. WebMaster - kalu@ucsd.edu , qqin@eng.ucsd.edu